HTB-126 Hs 578T 人乳腺癌細(xì)胞
ATCC® Number: HTB-126?
Designations: Hs 578T
Depositors: AJ Hackett
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: mammary gland; breast
Disease: carcinoma
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Receptors: estrogen receptor, not expressed [1119]
Tumorigenic: No
DNA Profile (STR): Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,12
D5S818: 11
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 17
Cytogenetic Analysis: Number of cells examined = 50; Modal Chromosome Number = 59 with a range of 50 to 77; Polyploidy Rate = 33.8%
Composite karyotype: 50-77 <3n> X, -1, del(1)(q12), -2, del(2)(?q36), der(3)t(3;15)(q10;p10),-4, -5,der(5)t(5;8)(p10;q10),-6, i(6)(p10), +8, -9, -10, -11, del(11)(p12), -12, -13, -14, -15, -15, -16, -17, -17, -17, i(17)(q10), -18, -19,der(19)(19pter<-q13::5q13<-qter), +22, +3 mar[cp12]
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 1
Age: 74 years
Gender: female
Ethnicity: Caucasian
Comments: The Hs 578T cell strain was derived from a carcinoma of the breast.
It was originated by A.J. Hackett, et al. along with the Hs 578Bst (see ATCC HTB-125), which is a normal fibroblast-like line from the same patient.
The Hs 578T line had a mixed polygonal morphology initially, but a stellate cell type was selected for during passage and by cloning.
Aggregates of casein protein granules, desmosomes, tight junctions, lipid droplets and vesicularized smooth endoplasmic reticulum were observed by electron microscopy.
As with Hs 578Bst, no estrogen receptors or endogenous viruses were detected.
Cytogenetics: Derivatives and Markers
There are 8 consistent derivative chromosomes: del(1)(q12), del(2)(?q36), der(3)t(3;15)(q10;p10), der(5)t(5;8)(p10;q10), i(6)(p10), del(11)(p12), i(17)(q10), der(19)(19pter<-q13::5q13<-qter) plus two markers of unknown origin and one minute chromosome.
Four other markers, including two derivative chromosome 1s were noted are lower frequency.
Cytogenetics: Comments
This is a hypotriploid human cell line with a modal chromosome number of 59.
QM staining verified the absence of a Y chromosome.
The rate of polyploidy in excess of the modal number is 33.8%.
There were 8 consistent derivative chromosomes: del(1)(q12), del(2)(?q36), der(3)t(3;15)(q10;p10),der(5)t(5;8)(p10;q10), i(6)(p10), del(11)(p12),i(17)(q10),
der(19)(19pter<-q13::5q13<-qter) plus two markers of unknown origin and one minute chromosome.
Normal chromosome 17's were absent and only a single normal 15 was seen in most cells.
No brightly fluorescent Y chromosomes were detected with QM staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
normal (or near-normal) cell line established from the same patient:ATCC HTB-125
purified DNA:ATCC HTB-126D
purified RNA:ATCC HTB-126R
References: 1119: Hackett AJ, et al. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs 578T) and the diploid myoepithelial (Hs 578Bst) cell lines. J. Natl. Cancer Inst. 58: 1795-1806, 1977. PubMed: 864756
22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258
32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764
