產(chǎn)品資料
產(chǎn)品[

SW1116 人結(jié)腸腺癌細胞

]資料
如果您對該產(chǎn)品感興趣的話,可以 sendmsg
產(chǎn)品名稱: SW1116 人結(jié)腸腺癌細胞
產(chǎn)品型號: CCL-233
產(chǎn)品廠商: 美國標準生物品收藏中心（ATCC）
產(chǎn)品文檔: 無相關(guān)文檔


簡單介紹

CCL-233 SW1116 人結(jié)腸腺癌細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！

SW1116 人結(jié)腸腺癌細胞 的詳細介紹
CCL-233 SW1116 人結(jié)腸腺癌細胞
ATCC® Number: CCL-233?     
Designations: SW1116 [SW 1116, SW-1116]
Depositors:  A Leibovitz
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
   
   
:  
 
   
   
   
   
   
Cytogenetic Analysis: modal number = 60; range = 50 to 62
The stemline chromosome number is hypotriploid with 2S component occurring at 2.8% and 9 markers were common to S metaphases. Neither HSR chromosomes nor DM were seen. Karyotypes were less variable between cells.
   
   
   
Ethnicity: Caucasian
Comments: CSAp negative (CSAp-).
Colon antigen 3, negative.
The cells are positive for keratin by immunoperoxidase staining.
The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes.
N-myc and N-ras oncogene expression were not detected.
Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Cells must be subcultured at about 80% confuency , before they reach 90%.
Protocol: 1. Remove and discard culture medium.

2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5. Add appropriate aliquots of the cell suspension to new culture vessels.

6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 1 to 2 times per week
:  
   
   

產(chǎn)品留言
標題
聯(lián)系人
聯(lián)系電話
內(nèi)容
驗證碼
點擊換一張
注：1.可以使用快捷鍵Alt+S或Ctrl+Enter發(fā)送信息!
2.如有必要,請您留下您的詳細聯(lián)系方式!

聯(lián)系我們
產(chǎn)品搜索

細胞系，ATCC細胞，ATCC菌種，細胞系目錄，ATCC，鼠傷寒沙門氏菌，atcc細胞庫，人**瘤細胞，人食管癌細胞，ATCC 19404，ATCC 25922，ATCC 19146，微生物菌種，細胞株，標準菌株，ATCC中國，atcc上海代理，CMCC 菌株，ATCC菌株，細胞目錄，質(zhì)控菌株，腫瘤細胞株，乳腺癌細胞系，人肝癌細胞系
產(chǎn)品目錄

Copyright? 2003-2025  上海復祥生物科技有限公司版權(quán)所有            電話：021-35080510
傳真：021-35080520    地址：上海市虹口區(qū)赤峰路630號南雁大廈1901室    郵編： 滬ICP備10013034號-3  

  

滬公網(wǎng)安備 31010902002429號