產品[
CRL-1572 PA-1 人卵巢畸胎瘤細胞
]資料
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產品名稱:
CRL-1572 PA-1 人卵巢畸胎瘤細胞
產品型號:
CRL-1572
產品廠商:
美國標準生物品收藏中心（ATCC）
產品文檔:
無相關文檔
簡單介紹
CRL-1572 PA-1 人卵巢畸胎瘤細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！
CRL-1572 PA-1 人卵巢畸胎瘤細胞
的詳細介紹
CRL-1572 PA-1 人卵巢畸胎瘤細胞
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CRL-1572?
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$318.00
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PA-1
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BC Giovanella
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: ovary
Disease: teratocarcinoma
Derived from metastatic site: ascites
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Yes
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N-ras + (activated)
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HLA A28, B12
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Amelogenin: X
CSF1PO: 9,12
D13S317: 9,10
D16S539: 9,12
D5S818: 11
D7S820: 9
THO1: 7,9
TPOX: 11
vWA: 15,17
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G6PD, B
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12 years
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female
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
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culture medium 95%; DMSO, 5%
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
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22179: . . In Vitro 10: 382, 1974.
22368: Zeuthen J, et al. Characterization of a human ovarian teratocarcinoma-derived cell line. Int. J. Cancer 25: 19-32, 1980. PubMed: 6931103
22530: Giovanella BC, et al. Heterotransplantation of human malignant tumors in "nude" thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. J. Natl. Cancer Inst. 52: 921-930, 1974. PubMed: 4524119
22950: Tainsky MA, et al. An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells. Science 225: 643-645, 1984. PubMed: 6740333
23112: McGowan-Jordan IJ, et al. Suppression of tumorigenicity in human teratocarcinoma cell line PA-1 by introduction of chromosome 4. Cancer Res. 54: 2568-2572, 1994. PubMed: 8168081
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