產(chǎn)品[
CRL1472 HT-1376人膀胱癌細(xì)胞
]資料
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產(chǎn)品名稱:
CRL1472 HT-1376人膀胱癌細(xì)胞
產(chǎn)品型號(hào):
CRL-1472
產(chǎn)品廠商:
美國標(biāo)準(zhǔn)生物品收藏中心(ATCC)
產(chǎn)品文檔:
無相關(guān)文檔
簡單介紹
CRL-1472 HT-1376 人膀胱癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*優(yōu)培養(yǎng)條件!
CRL1472 HT-1376人膀胱癌細(xì)胞
的詳細(xì)介紹
CRL-1472 HT-1376 人膀胱癌細(xì)胞
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CRL-1472?
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$323.00
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HT-1376
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S Rasheed
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Biosafety Level:
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1
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: urinary bladder
Tumor Stage: grade 3
Disease: carcinoma
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fibrinolytic activity; interferon
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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transfection host (Roche FuGENE® Transfection Reagents)
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Yes
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negative
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Amelogenin: X
CSF1PO: 12
D13S317: 9,11
D16S539: 11,14
D5S818: 11,12
D7S820: 9,12
THO1: 7,10
TPOX: 8
vWA: 15,18
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modal chromosome number = 104 to 121
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G6PD, B
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58 years
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female
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks.
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purified DNA:ATCC CRL-1472D
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22538: Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628
23134: Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805
25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484
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