如果您對(duì)該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱:
人 乳腺細(xì)胞
產(chǎn)品型號(hào):
CRL-2713 MDA-kb2 人乳腺細(xì)胞
產(chǎn)品廠商:
美國(guó)標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔:
無(wú)相關(guān)文檔
簡(jiǎn)單介紹
CRL-2713 MDA-kb2 人乳腺細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|。細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*優(yōu)培養(yǎng)條件!
人 乳腺細(xì)胞
的詳細(xì)介紹
CRL-2713 MDA-kb2 人乳腺細(xì)胞
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CRL-2713?
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MDA-kb2
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VS Wilson
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Biosafety Level:
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1
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: mammary gland; breast
Cell Type: epithelial
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luciferase ( [57526] The cell line expresses firefly luciferase under control of the MMTV promoter that contains response elements for both glucocorticoid receptors (GR) and androgen receptors (AR).)
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation date: November 25, 1998
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assay of androgen receptor ( [57526] MDA-kb2 may be used in an in vitro assay to screen androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine disrupting chemicals.)
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androgen receptor [57526]
glucocorticoid receptor, positive [57526]
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Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 6
TPOX: 10
vWA: 17,18
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48 years *****
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female
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
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Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
parental cell line:ATCC HTB-131
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57526: Wilson VS, et al. A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists. Toxicol. Sci. 66: 69-81, 2002. PubMed: 11861974
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